Open AccessIsolation and Characterization of Antioxidant Active Compounds from the Leaves and Flowers of Manilkara hexandra (Roxb.)
Author(s) -
T. Annamalai,
A.A.M. Prince
Publication year - 2021
Publication title -
asian journal of chemistry/asian journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.145
H-Index - 34
eISSN - 0975-427X
pISSN - 0970-7077
DOI - 10.14233/ajchem.2021.23355
Subject(s) - chemistry , gallic acid , antioxidant , fractionation , quercetin , flavonoid , methyl gallate , staphylococcus epidermidis , chromatography , traditional medicine , staphylococcus aureus , botany , organic chemistry , bacteria , biology , medicine , genetics
Fractionation and chromatographic purification of the crude methanol extract of air-dried leaves ofManilkara hexandra (Roxb) yielded five compounds p-methoxy methyl cinnamate (1),p-hydroxymethylcinnamate (2), 2,5-dihydroxybenzaldehyde (3), gallic acid methyl ester (4) andquercetin-3-O-β-D-glucoside (isoquercitrin) (5), whereas air-dried flowers yield phenylethylpalmitate(6). The structures of these compounds were confirmed using spectroscopic data, comparison withliterature data and co-TLC comparison with authentic samples. To the best of our knowledge compounds1, 3, 5 and 6 were isolated for the first time from this plant. Constituents of the plant were known to beactive against Staphylococcus aureus and Staphylococcus epidermidis. Comparative antioxidant activityof compounds along with the crude extract and fractions were assessed using four different assayprotocols. These compounds show moderate activity in the antioxidant assay systems. Present resultsrationalize the usage of the leaves and flower paste as bathing aid as suggested in the folklore literatureto maintain a dynamic balance of skin microflora.