Open AccessDevelopment and Validation of Highly Sensitive Spectrophotometric Methods for Cefpirome Determination in Pharmaceutical Dosage Forms
Author(s) -
Basavaraj Hiremath
Publication year - 2021
Publication title -
asian journal of chemistry/asian journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.145
H-Index - 34
eISSN - 0975-427X
pISSN - 0970-7077
DOI - 10.14233/ajchem.2021.23287
Subject(s) - chemistry , cefpirome , molar absorptivity , dosage form , chromatography , diphenylamine , absorption (acoustics) , organic chemistry , antibiotics , biochemistry , physics , antibiotic resistance , imipenem , acoustics , optics
Quantitative spectrophotometric determination of cefpirome in pure and pharmaceutical dosage hasbeen developed. Method I produces a pink-coloured chromogen peak at λmax 510 nm by reactingdiazotized cefpirome drugs with diphenylamine (DPA) in a neutral medium. Method II obtained of acoloured Schiff bases when cefpirome reacts with alcoholic p-dimethylaminobenzaldehyde (PDAB)to produce a yellow-coloured chromogen with a maximum absorption wavelength of 415 nm. In bothmethods I and II, Beer’s law is followed in the concentration ranges of 0.3-3.0 and 0.5-5.0 μg/mL,respectively, with molar absorptivity of 5.13 × 104 and 2.54 × 104 for each form. At three separateconcentrations, intra-day and inter-day (RSD) and relative error (RE) are measured. The current methodsare simple, reliable, inexpensive, speedy and highly reproducible and have been tested in broad rangeof pharmaceutical formulations with statistical comparisons to reference methods.