Antithrombotic, Antihemolytic Activities and Protein Conjugation Properties of Silver Nanoparticles Synthesized from Turbinaria ornata

Green synthesis of silver nanoparticles synthesized from Turbinaria ornata weed extract was carriedout. Because these seaweeds are largely unexplored and contain botanical molecules that promotebimolecular reduction. In this study, silver nanoparticles (AgNPs) cause a rise in the accumulation ofclotting factors, platelets and procoagulant activity, both of which lead to thrombotic and hemolyticdiseases. The silver nanoparticles were initially characterized using spectroscopic analysis to validatethe biomolecules of Turbinaria ornata involved in nanoparticle reduction. A detailed study on theeffectiveness of silver nanoparticles on antithrombolytic activity in adult blood samples confirms theinteraction of nanoparticles with platelets and blood vessels, which is significant in the production ofthrombosis and cardiovascular diseases. The antihemolytic activity was tested to assess the percentageof blood clot lysis in order to confirm the ability to scavenge hydrogen peroxide in a concentrationdependent manner. The protein conjugation and binding activities of major, secondary, and tertiarystructures were also investigated in silico. Thus, Turbinaria ornata seaweeds with reduced silvernanoparticles demonstrated increased antithrombotic and antihemolytic activities, suggested that theycould be used as novel therapeutics for cardiovascular diseases.