Open AccessNanoparticles Mediated Protein Stability in Comparison with Osmolytes: in vivo Approach
Author(s) -
Ritu Verma,
Neetu Singh,
Pratima Chaudhuri
Publication year - 2021
Publication title -
asian journal of chemistry/asian journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.145
H-Index - 34
eISSN - 0975-427X
pISSN - 0970-7077
DOI - 10.14233/ajchem.2021.23219
Subject(s) - osmolyte , chemistry , protein stability , in vivo , nanoparticle , protein folding , dihydrofolate reductase , colloidal gold , biophysics , biochemistry , nanotechnology , enzyme , biology , microbiology and biotechnology , materials science
The native three-dimensional structure of protein is quite unstable under critical destabilizing conditions.In order to enhance the stability and activity for a proper folded environment of a protein, manystabilizing materials are added such as nanoparticles and osmolytes to an unfolded state of protein.Osmolytes are the important group of molecules which are engaged by the cell as an adaption in thesevere conditions. In this communication, a comparative in vivo study is reported for imparting thestatus of stability and folding ability of zebrafish dihydrofolate reductase (zDHFR) protein with goldnanoparticles and various osmolytes (glycerol, glucose and betain). Present observations revealed thatthe interaction of gold nanoparticles (AuNPs) with bacteria at the cellular level helps in maintainingthe stability of protein more effectively than osmolytes which could be used for many biological andpharmacological approaches although glycerol as an osmolyte also stabilizes the protein at a significantlevel.