Open AccessIdentification of Bioactive Compounds from Alphonsea madraspatana Leaves against Multi Drug Resistance Bacteria
Author(s) -
Ankit Kumar Sahu,
Shekhar Swain Sudhanshu,
Deepak Pradhan,
Dipak Kumar Sahu,
Prativa Biswasroy,
Biswakanth Kar,
Goutam Rath,
Goutam Ghosh
Publication year - 2021
Publication title -
asian journal of chemistry/asian journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.145
H-Index - 34
eISSN - 0975-427X
pISSN - 0970-7077
DOI - 10.14233/ajchem.2021.23160
Subject(s) - antimicrobial , chemistry , kaempferol , chromatography , hexane , glycoside , traditional medicine , flavonoid , stereochemistry , organic chemistry , antioxidant , medicine
In this study, the antimicrobial compounds present in leaves of Alphonsea madraspatana was identifiedby using RP-HPLC, LC/MS and GC/MS and found to be active against multiple drug resistant (MDR)bacteria e.g. Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa. Extraction ofdried plant leaves was performed by solvent gradient technique. Antimicrobial study was performedwith each extract. n-Hexane and methanol extracts were further subjected to analysis for identificationof potent antimicrobial compounds due to their better antimicrobial activity as compared to remainingextracts. The methanolic extract was more active than n-hexane extract. As n-hexane extract containsnon-polar compounds, it was subjected to GC-MS analysis and methanol extract was subjected to RPHPLCfollowed by LC/MS due to presence of polar components. Among the isolated compounds,four compounds were identified as potent antimicrobial. RP-HPLC and LC/MS analysis of methanolextract also ensured the presence of four potent antimicrobial compounds as follows: (i)kaempferol-3-O-rotinoside-orhamnoside (m/z 748), (ii) 3-hydroxypropylglucosinolate (m/z 377), (iii)luteolin-7-O-glucoside (m/z 448) and (iv) genistein-7-O-glycoside (m/z 432). Leaves of this plant maybe used as potent antibacterial agents due to presence of antimicrobial compounds.