Open AccessAnti-inflammatory and Antioxidant Activities of Teucrium polium Leaf Extract and its Phenolic and Flavonoids Content
Author(s) -
Khaled Qabaha,
Thameen Hijawi,
Abdallah Mahamid,
Haneen Mansour,
Naeem Ahmad,
Jehad Abbadi,
Fuad AlRimawi
Publication year - 2021
Publication title -
asian journal of chemistry/asian journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.145
H-Index - 34
eISSN - 0975-427X
pISSN - 0970-7077
DOI - 10.14233/ajchem.2021.23096
Subject(s) - teucrium , antioxidant , chemistry , polyphenol , traditional medicine , anti inflammatory , tumor necrosis factor alpha , flavonoid , food science , biochemistry , pharmacology , biology , lamiaceae , medicine , immunology
Teucrium polium leaf is one of the folkloric medicinal plants used traditionally to treat many diseasesin the Palestinian community. It has antibacterial antioxidant and anti-inflammatory consequences.Present study aims the evaluating the in vitro inhibitory effect of Teucrium polium leaf extracts ontumor necrosis factor-α (TNF-α) using polymorphonuclear cells (PMNCs), in addition to determineits antioxidant and total phenolic and flavonoids contents. Polymorphonuclear cells were withdrawnfrom whole blood according to Histopaque (Ficol-1077) method. Blood cells were cultured in anenriched Roswell Park Memorial Institute (RBMI) medium. The levels of tumor necrosis factor (TNF-α)were determined after 24 h using LPS stimulation. Total phenolic contents, flavonoids contents andantioxidant activity were measured using spectrophotometric method. The TNF-α concentrations werecompared using paired-samples t test. The leaf extracts of Teucrium polium revealed significantreduction in terms of TNF-α levels. The extract contained high phenolic and flavonoids contents andits antioxidant activities were remarkable. The reduced values in the TNF-α levels as affected byTeucrium polium leaf extracts indicate its effect in anti-inflammation. The plant is rich with polyphenoliccompounds and flavonoids and has strong antioxidant activity. The observed anti-inflammatory effectof the extracts under study may be discussed as the influence of the significant presence of the phenoliccompounds and flavonoids.