Open AccessSimultaneous Estimation of Aflatoxins (B1, B2, G1 and G2) by Liquid Chromatography Coupled with Mass Spectrometry (LC-MS) in Corn Samples
Author(s) -
O. Sailaja,
M. Manoranjani,
G. Krishnaveni
Publication year - 2021
Publication title -
asian journal of chemistry/asian journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.145
H-Index - 34
eISSN - 0975-427X
pISSN - 0970-7077
DOI - 10.14233/ajchem.2021.23031
Subject(s) - aflatoxin , chemistry , contamination , chromatography , detection limit , liquid chromatography–mass spectrometry , mycotoxin , food science , high performance liquid chromatography , food contaminant , mass spectrometry , biology , ecology
Aflatoxins produced by Aspergillus flavins and Aspergillus pasasiticus have been investigated in thecorn (maize) collected in different locations in Andhra Pradesh state, India. In the present study, 24corn samples collected from different harvest, storage and dumping areas were analyzed for detectionof aflatoxin contamination by liquid chromatography coupled with mass spectrometry (LC-MS). Mixedaflatoxin working standard solutions with a concentration of 10, 20, 30, 40, 50 and 60 ng/mL wereprepared and followed by instrumental analysis and the retention times of the sample chromatogramswere compared with the standard aflatoxins B1, B2, G1 and G2 retention times in order to determine thecontamination. The findings revealed that among 24 collected samples, only 10 samples have beenfound positive for aflatoxins contamination when subjected to LC-MS analysis. Co-occurrence ofaflatoxin was identified in the most of the samples. Even though 10 samples were tested positive totalaflatoxin content in the most positive are found within the acceptable limits (30 μg/kg) and samplescollected at dumping areas (S4, S8 and S10) show the presence of exceeding quantity than the limitsdescribed by FSSAI. Total aflatoxin content in the most positive were found within the acceptablelimits (30 μg/kg) and the samples collected at dumping areas S4 (40.9 μg/kg), S8 (53.2 μg/kg) andS10 (71.86 μg/kg) had shown exceeding limits. The sample S7 collected from the storage was alsofound positive of presence of aflatoxin G2- 13.49 μg/kg and B2-21.14 μg/kg with total aflatoxin of 34.65μg/kg. High contamination of these dumping areas may lead to contamination of storage points nearerto these areas. Present results showed that the corn samples at dumping areas were found to becontaminated with the Aspergillus flavins and Aspergillus pasasiticus.