Open AccessVariations in Andrographolide Content, Phytochemical Constituents and Antioxidant Activity of Leaves of Andrographis paniculata (L.) Nees Collected from Different Locations of Southern India
Author(s) -
G. Vinayak,
Praveen Nagella
Publication year - 2021
Publication title -
asian journal of chemistry/asian journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.145
H-Index - 34
eISSN - 0975-427X
pISSN - 0970-7077
DOI - 10.14233/ajchem.2021.23020
Subject(s) - andrographolide , chemistry , andrographis paniculata , chloroform , phytochemical , flavonoid , acanthaceae , traditional medicine , methanol , antioxidant , food science , chromatography , biochemistry , medicine , alternative medicine , organic chemistry , pathology
In present study, the samples collected from different locations of Southern India viz., YellapurBeltargadde, Siddapur, Joida, Ankola, Sirsi Kangod, Yellapur Shalabail, Sirsi Bairumbe, Karwar ofKarnataka and Kasaragod from Kerala were analyzed for the andrographolide content, total phenolicand flavonoid content and screened for their antioxidant potential. The A. paniculata leaves wereextracted with three different solvents (chloroform, methanol and water) and methanolic extract ofSiddapur showed highest (8.82 ± 0.25 mg/g DW) amount of phenolic content whereas, aqueous extractof Ankola (3.00 ± 1.18 mg/g DW) showed the least amount. Chloroform extract of Yellapur Beltargaddevillage showed highest quantity i.e. 1.87 ± 0.50 mg/g DW of flavonoid content and aqueous extract ofYellapur Beltargadde showed 0.30 ± 0.20 mg/g DW which was least among all the tested samples.The sample collected from Karwar was found to have highest andrographolide content (9.36 ± 0.02mg/g DW) followed by Yellapur Beltargadde sample with 7.29 ± 0.01 mg/g DW and Sirsi Kasaragodhas the lowest contents of 1.54 ± 0.1 mg/g DW when analyzed through HPLC. Among the ninelocations, methanol extract from Joida showed highest percentage of scavenging activity (91.95%)followed by methanol extract of Ankola (90.42%) and chloroform extract of Siddapur (77.31%) whichwas the lowest value of all samples tested.