Open AccessAverrhoa bilimbi: A Prospective Source of Bioactive Compounds against Antimicrobial and Cytotoxic Activities
Author(s) -
Md. Abdul Mannan,
Liton Chandra,
Arshad Islam,
Saddam Hossain,
Md. KudratEZahan,
Tetsuya Kida
Publication year - 2020
Publication title -
asian journal of chemistry/asian journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.145
H-Index - 34
eISSN - 0975-427X
pISSN - 0970-7077
DOI - 10.14233/ajchem.2021.22933
Subject(s) - chemistry , antimicrobial , petroleum ether , ethyl acetate , dpph , brine shrimp , flavonoid , chloroform , food science , chromatography , antioxidant , traditional medicine , organic chemistry , extraction (chemistry) , medicine
Averrhoa bilimbi fruit has been studied to find out a possible source of bioactive compounds againstantimicrobial and cytotoxic activity. To achieve this goal, total phenolic content and total flavonoidcontent, antioxidant, antimicrobial and cytotoxic activities of five different fractionates such asmethanolic crude, petroleum ether, chloroform, ethyl acetate and water fraction of A. bilimbi wereinvestigated by UV-vis spectrophotometry, disc diffusion and brine shrimp lethality test methodssimultaneously. Other phytochemicals viz. carbohydrate, tannins, polyphenols, glycosides, etc. werealso screened out by conventional test methods concurrently. Total phenolic contents and total flavonoidcontents were found to be ranging from 3.807-262.129 mg GAE g–1 and 67.848-1159.061 mg CE g–1,respectively, in all the analyzed fractions. DPPH free radical scavenging activity revealed distinctiveIC50 value of 5.63 and 10.26 μg mL–1 in the chloroform and ethyl acetate extractives. Fractionates ofpetroleum ether and methanolic crude also presented strong cytotoxic potential with the LD50 valuesof 8.89 and 20.3 μg mL–1 in the brine shrimp lethality bioassay test. Petroleum ether and ethyl acetatefractions exhibited good antimicrobial activity with the zone of inhibition against Bacillus cereus (11mm and 14 mm) and Escherichia coli (7 mm and 9 mm). Finally, it has been recommended that A.bilmbi fruit might be an effective source of bioactive compounds against antimicrobial and cytotoxicactivity as well as antioxidant with potent radical scavenging activity.