Open AccessScreening, Identification and Controlling Effect of Antifungal Compound from Bacillus megaterium LB01-16
Author(s) -
Luo-Qing Wei,
Cong-Wen D ing,
Shang-Ze Zhou
Publication year - 2020
Publication title -
asian journal of chemistry/asian journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.145
H-Index - 34
eISSN - 0975-427X
pISSN - 0970-7077
DOI - 10.14233/ajchem.2020.22942
Subject(s) - bacillus megaterium , postharvest , colletotrichum gloeosporioides , spore germination , chemistry , mycelium , fermentation , antifungal , food science , spore , botany , microbiology and biotechnology , biology , bacteria , genetics
In order to screen, identify high active compound and investigate its controlling effect on anthracnosedisease in postharvest mangos, the bioassay-guided fractionation was used to screen and isolate antifungalcomponents from fermented broth of Bacillus megaterium LB01-16 and the inhibition zone diameter ofactive components isolated against Colletotrichum gloeosporioides were measured with a disk diffusiontest. The results revealed that four monomer compounds were separated from the fermentation broth,and only one monomer with strong antifungal activity, numbered 007, was obtained and identified asglucopyranosyl 12-hydroxyjasmonic acid by 1H & 13C NMR and by comparing with literature. Thisstudy confirmed that the glucopyranosyl 12-hydroxyjasmonic acid produced from Bacillus megateriumLB01-16 can inhibit the anthracnose disease of postharvest mangos by directly reducing myceliumgrowth and inhibiting the spore germination of Colletotrichum gloeosporioides, thus providing apromising strategy for controlling of anthracnose disease in postharvest mangos.