Open AccessEvaluation of Protein Microencapsulation Efficiency in Alginate/Hydroxyethyl Cellulose Polymer Composite
Author(s) -
Rodel D. Guerrero,
Paul Wan Sia Heng,
Terence Tumolva
Publication year - 2020
Publication title -
asian journal of chemistry/asian journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.145
H-Index - 34
eISSN - 0975-427X
pISSN - 0970-7077
DOI - 10.14233/ajchem.2020.22896
Subject(s) - chemistry , hydroxyethyl cellulose , polymer , bovine serum albumin , composite number , chemical engineering , coating , cellulose , box–behnken design , ionotropic effect , chromatography , response surface methodology , materials science , composite material , organic chemistry , biochemistry , engineering , nmda receptor , receptor
Considering how much protein is loaded into the process, the crosslinking solution concentration and the weight percentage of thealginate/hydroxyethyl cellulose coating material, this study presented the details of optimization of the microencapsulation process.Optimization was done with an objective of maximizing the encapsulation efficiency of the microparticle fabricated using externalionotropic gelation method. Results showed that the process is capable of achieving around 75% encapsulation efficiency when proteinloading is around 15 wt%, alginate/hydroxyethyl cellulos is about 2 wt% and the CaCl2 solution should be 3 wt%. This was done usingBox-Behnken methodology wherein the predicted model was found to have good predictive capability. Analysis also showed that theprocess is affected by how much protein drug is loaded into the system and the interactions between crosslinking solution concentration withbovine serum albumin (BSA) loading as well as the strong relationship between alginate/hydroxyethyl cellulos concentration with itself.