Open Accessin vitro Antioxidant Activity, Total Phenolic Content and Antimicrobial Activity of Coleus forskohlii Grown in Al-Baha, Saudi Arabia
Author(s) -
Abdelfatah A. Fadlelmula,
Abdulaziz Yahya Al-Ghamdi,
Mohamed Abdalla
Publication year - 2020
Publication title -
asian journal of chemistry/asian journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.145
H-Index - 34
eISSN - 0975-427X
pISSN - 0970-7077
DOI - 10.14233/ajchem.2020.22716
Subject(s) - petroleum ether , coleus , chemistry , ethyl acetate , chloroform , antimicrobial , ethanol , traditional medicine , antioxidant , butanol , food science , chromatography , organic chemistry , extraction (chemistry) , botany , biology , medicine
In this study, the active ingredients from Coleus forskohlii were detected and evaluated their antioxidantand antimicrobial activities. Coleus forskohlii leaves were collected, air dried, powdered and extractedfirst with aqueous ethanol and secondly with petroleum ether, chloroform, ethyl acetate and n-butanolsuccessively. The chemical analysis of the extracts showed the existence of (flavonoids, alkaloids,tannins, terpenoids, steroids, saponins and sugars). Furthermore, the extracts were subjected to analysisof total phenolic contents. Where the n-butanol exhibited highest TPC value (384.23±15.86 mg GAE/g),followed by ethyl acetate (199.73±29.35 mg GAE/g) then petroleum ether (96.97±61.29 mg GAE/g),ethanol (90.80±17.51 mg GAE/g), and eventually chloroform extract exhibited the lowest (TPC) value.In addition to that the antioxidant status of the different extracts of Coleus forskohlii was detected.Butanolic extract showed the highest radical scavenging (96.33±1.53 %, lowest IC50 value 0.03) followedby ethyl acetate extract (46.67±3.50 %, IC50 value 0.07). Eventually the antimicrobial activity testswere carried out for all the plant extracts. Where n-butanol showed the maximum inhibition (14±0.70mm). All the pathogens except C. albicans exhibited moderate response towards petroleum etherextract. C. albicans was sensitive towards ethanol extract only (14.00±1.41), whereas the otherGram-positive and Gram-negative bacteria exhibited high impedance to the ethanol, chloroform, andethyl acetate extracts. This study scientifically props the usage of whole plant as a medicine for severalsurface bacterial and fungal strike in folk medicine, in addition to this the plant may serve as a exporterfor additional development of indigenous antioxidant and antitumor agents.