Open AccessFractionation and Identification of Phytochemicals and Antioxidant Activity of Wild Grape (Ampelocissus martinii Planch.) Seed Extracts
Author(s) -
Patthraporn Siripipatthana,
Prasong Srihanam
Publication year - 2020
Publication title -
asian journal of chemistry/asian journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.145
H-Index - 34
eISSN - 0975-427X
pISSN - 0970-7077
DOI - 10.14233/ajchem.2020.22662
Subject(s) - chemistry , ethyl acetate , gallic acid , phytochemical , antioxidant , myricetin , fractionation , food science , catechin , phenols , polyphenol , flavonoid , chromatography , biochemistry , kaempferol
A methanolic extract of wild grape (Ampelocissus martinii Planch.) seed at green (immature) growthstage was partially purified using silica gel chromatography, before the investigation of totalphytochemicals and antioxidant activity of sub-fractions. The ethyl acetate/methanol at 3:1 (v/v) fractionhad higher phytochemicals and antioxidant activities than others. The gallic acid, myricetin, andresveratrol were dominant phenolic substances in this fraction. However, ethyl acetate sub-fractionfound the highest content of epicatechin and catechin. A correlation test indicated a strong positivetrend between antioxidant activity and all tested of the phytochemical contents. The obtained resultsindicated that the wild grape seed contains compounds with excellent antioxidation capacity. Thatmight be applied as an active ingredient in health-supporting products or active ingredients in cosmetics