Open AccessGreen Synthesis and Evaluation of Dimocarpus longan Leaves Extract Based Chitosan Nanoparticles against Periodontitis Triggering Bacteria
Author(s) -
Neeraj Kumar Fuloria,
Mok Yong Ko,
Chuang Rui,
Chin Zi Hang,
Kalyana Sundram,
Neeraj Paliwal,
Usha Kumari,
K. C. Gupta,
Kathiresan V. Sathasivam,
Shivkanya Fuloria
Publication year - 2020
Publication title -
asian journal of chemistry/asian journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.145
H-Index - 34
eISSN - 0975-427X
pISSN - 0970-7077
DOI - 10.14233/ajchem.2020.22649
Subject(s) - chemistry , nuclear chemistry , chitosan , distilled water , phytochemical , absorbance , tannic acid , food science , chromatography , organic chemistry , biochemistry
Present study was intended to synthesize and evaluate the Dimocarpus longan leaves aqueous extract(DLLAE) based chitosan nanoparticles (CSNPs) against periodontitis triggering bacteria. In presentstudy the DLLAE was prepared by extracting D. Longan leaves in distilled water using decoctionmethod. The prepared DLLAE was subjected to phytochemical investigations, total phenolic content,total flavonoid content study and CSNPs synthesis. The CSNPs were characterized (using UV-visible,FT-IR, SEM, FESEM, XRD and EDX) and evaluated for their potential against periodontitis triggeringbacteria (using agar well diffusion method). The phytochemical investigations over DLLAE exhibitedpresence of monosaccharides, reducing sugars, proteins, steroids, anthraquinones and coumarinsglycosides, flavonoids, alkaloids, organic acid, tannic and phenolic compounds. The CSNPs wereformed using DLLAE when the colour changed from white to pale brown. Absorbance of the CSNPswas in the desired range (201-310 nm). The optimization study revealed 1% acetic acid, 1% sodiumalginate, 2 mg/mL chitosan, pH 5.8 and 1 mL DLLAE as the standard requirement for CSNPs synthesisusing DLLAE. The CSNPs characterization data of FT-IR exhibited shifted bands. The FESEM revealedthat the synthesized CSNPs were spherical in shape and their size ranged from 74.66 to 94.07 nm. TheXRD data showed peaks at 2θ values of 22.28º, 25.19º, 34.72º, 40.0º, 71.16º and 73.0º. The averagesize for CSNPs was between 30.11-62.98 nm. The EDX spectrum revealed signal for carbon (76.17%),oxygen (22.14%) and sodium (1.53%) which confirmed formation of CSNPs. The antibacterial activityrevealed high inhibitory potential of green CSNPs against S. aureus. Present studies establish thatCSNPs biosynthesis using D. longan leaves aqueous extract is an efficient method and thebiosynthesized CSNPs possess high antibacterial potential against S. aureus (bacteria that triggersperiodontitis).