Open AccessRP-HPLC Method for Determination of Salbutamol and Bromhexine in Syrup: Modelling and Optimization by Response Surface Methodology
Author(s) -
Chung Duong Dinh,
Yen Nguyen Ngoc Thi,
Khanh Quan Nguyen Huu,
Duy Chinh Nguyen,
Ung Thanh Dat,
Thuy Ca Thi
Publication year - 2020
Publication title -
asian journal of chemistry/asian journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.145
H-Index - 34
eISSN - 0975-427X
pISSN - 0970-7077
DOI - 10.14233/ajchem.2020.22635
Subject(s) - chemistry , chromatography , central composite design , salbutamol , response surface methodology , detection limit , high performance liquid chromatography , correlation coefficient , volumetric flow rate , capacity factor , analytical chemistry (journal) , mathematics , thermodynamics , statistics , medicine , physics , asthma
In present work, the RP-HPLC method was established for the determination of bromhexine andsalbutamol in syrup by using a design of experiment approach. The Plackett-Burman design wasapplied to screen the influence of independent variables (ratio of organic solvent and pH in mobilephase, flow rate, column temperature, sample injection volume and detection wavelength) on theoutput data of chromatographic signals (peak area, tailing factor, theoretical plates, resolution) ofbromhexine and salbutamol. The Pareto diagram shows that the selected variables affect mainly targetfunction. A central composite design has been used to optimize the values of main factors and Designexpert® software predicts the interaction and quadratic model to evaluate the impact of input parameterson output. The optimal conditions were determined with the support of response surface methodologyfor flow rate 0.9 mL/min, temperature 25 °C and 60% methanol in water with 0.06% orthophosphoricacid as the mobile phase. Good linearity was observed in the concentration range of 8-48 μg/mL forbromhexine and 4-24 μg/mL for salbutamol with a significantly high correlation coefficient (R >0.999). The limit of detection and limit of quantitation were 0.32 and 0.96 μg/mL, respectively forbromhexine and 0.08 and 0.25 μg/mL, respectively for salbutamol. This method was validated accordingto ICH guidelines.