Open AccessCytotoxic Sterols from Philippine Mushrooms
Author(s) -
Consolacion Y. Ragasa,
Glenn G. Oyong,
Maria Carmen S. Tan,
Mariquit M. De Los Reyes,
Maria Ellenita G. de Castro
Publication year - 2020
Publication title -
asian journal of chemistry/asian journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.145
H-Index - 34
eISSN - 0975-427X
pISSN - 0970-7077
DOI - 10.14233/ajchem.2020.22591
Subject(s) - chemistry , ic50 , cytotoxicity , cytotoxic t cell , ergosterol , sulforhodamine b , cell culture , mtt assay , cell , biochemistry , in vitro , biology , genetics
Ergosterol peroxide (1) and ergosterol (2) were commonly isolated as the major compounds of Philippinemushrooms. Sterols 1 and 2 from the dichloromethane extract of Geastrum triplex and Termitomycesclypeatus, respectively, were evaluated for their cytotoxic activities against four human cancer celllines, viz., breast cancer (MCF-7), colon cancer (HT-29), leukemia (THP-1), and small lung cellcarcinoma (H69PR), and a human normal cell line, human dermal fibroblast-neonatal (HDFn), usingthe PrestoBlue® cell viability assay. Compounds 1 and 2 exhibited the strongest activities againstHT-29 with IC50 values of 1.79 and 2.98 μg/mL, respectively, while Zeocin gave an IC50 of 4.89μg/mL. These compounds also exhibited strong antiproliferative effects against MCF-7 with IC50values of 4.13 for 1 and 4.20 μg/mL for compound 2, comparable to Zeocin with IC50 = 3.68 μg/mL.Only moderate cytotoxicity resulted when compounds 1 and 2 were tested against H69PR with IC50values of 7.78 and 6.83 μg/mL, respectively, while Zeocin exhibited an IC50 of 9.81 μg/mL. Furthermore,compounds 1 and 2 showed no effects against THP-1 (IC50 > 100 μg/mL), while Zeocin showed anIC50 of 4.73 μg/mL. Although compounds 1 and 2 have been reported to exhibit different bioactivitiesin previous studies, the cancer cell lines tested and/or the polarities of the solvents for extractionvaried. Therefore, comparisons of the cytotoxic activities of compounds 1 and 2 with earlier studiescould not be made extensively.