Open AccessGreen Synthesis, Characterization and Antimicrobial Activity of Zinc Oxide Nanoparticles on Clinical Isolates of Streptococcus pyogenes
Author(s) -
S. Akash,
N. Ahalya,
P Dhamodhar
Publication year - 2020
Publication title -
asian journal of chemistry/asian journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.145
H-Index - 34
eISSN - 0975-427X
pISSN - 0970-7077
DOI - 10.14233/ajchem.2020.22495
Subject(s) - wurtzite crystal structure , nanoparticle , zinc , fourier transform infrared spectroscopy , chemistry , band gap , scanning electron microscope , spectroscopy , analytical chemistry (journal) , nuclear chemistry , materials science , chemical engineering , nanotechnology , organic chemistry , optoelectronics , quantum mechanics , engineering , composite material , physics
Zinc oxide nanoparticles were synthesized using epicarp of Punica granatum by combustion method at moderate temperatures. Zinc oxide nanoparticles obtained in agglomerate form were characterized by powder X-ray diffractometer (PXRD) and found to have hexagonal phase, wurtzite structure. The crystalline size of nanoparticle was found to be ~ 60 nm by using Debye-Scherrer formula. The morphology Index, Lorentz factor and Lorentz polarization factor were also calculated. Ultraviolet-visible spectroscopy (UV-vis) spectrum for ZnO nanoparticle showed a strong absorbanceat 374 nm. This corresponds to the calculated band gap energy of 3.48 eV and the particle size calculated using band gap was found to be 50 nm. The Fourier Transform Infrared Spectroscopy (FTIR) spectrum showed a peak at 499 cm-1, which indicated Zn-O stretch bond. The scanning electron microscopy (SEM) analysis proved the size of nanoparticles synthesized were around 50 nm and energy dispersive X-ray spectroscopy (EDS) revealed the elemental composition of zinc oxide nanoparticles. The ZnO-NPs were evaluated for antibacterial activity against gram positive, tonsillitis causing Streptococcus pyogenes. From the present study, it was concluded that zinc oxide nanoparticles synthesized by combustion method could be valuable and economic in the field of nanotechnology.