Open AccessValidated Stability Indicating HPLC Method for Simultaneous Quantification of Trithioparamethoxy Phenylpropene and Chlorpheniramine Maleate in Tablet Forms
Author(s) -
T. V. Sravanthi,
N. Madhavi
Publication year - 2020
Publication title -
asian journal of chemistry/asian journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.145
H-Index - 34
eISSN - 0975-427X
pISSN - 0970-7077
DOI - 10.14233/ajchem.2020.22487
Subject(s) - chlorpheniramine maleate , chemistry , chromatography , high performance liquid chromatography , hydrolysis , organic chemistry
A novel easy stability indicating high performance liquid chromatography method has been developedfor the concurrent assessment of trithioparamethoxy phenylpropene in combination withchlorpheniramine maleate using Luna C8 column with UV detection at 224 nm. The mobile phasecomprised of 0.02 N phosphate buffer (pH 5.5) and acetonitrile (55:45 v/v) was delivered with a flowrate of 1.5 mL/min. The method was linear over the concentration range 6.25-18.75 μg/mL(trithioparamethoxy phenylpropene) and 1.5-4.5 μg/mL (chlorpheniramine maleate). The limit ofquantification was 1.57 μg/mL (trithioparamethoxy phenylpropene) and 0.969 μg/mL(chlorpheniramine maleate). The calculated recoveries were 100.083-100.287% (trithioparamethoxyphenylpropene) and 99.827-100.277% (chlorpheniramine maleate). Trithioparamethoxy phenylpropeneand chlorpheniramine maleate were subjected to forced stress like acid hydrolysis, base hydrolysis,thermal and oxidation degradation. The drugs were found to degrade in the applied conditions. Thedegraded products were resolved effectively from the trithioparamethoxy phenylpropene andchlorpheniramine maleate. The suggested stability-indicating HPLC method can be used for thequantitative evaluation of trithioparamethoxy phenylpropene and chlorpheniramine maleate in bulkmedications and tablet formulations.