Open AccessBiosynthesis and Characterization of Bioplastic Poly-3-hydroxybutyrate from Hydrolyzate of Ulva using Bacillus subtilis rnM
Author(s) -
T. Revadhi,
R. Nanthini
Publication year - 2019
Publication title -
asian journal of chemistry/asian journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.145
H-Index - 34
eISSN - 0975-427X
pISSN - 0970-7077
DOI - 10.14233/ajchem.2019.22251
Subject(s) - bioplastic , bacillus subtilis , chemistry , hydrolysate , biosynthesis , food science , polyhydroxyalkanoates , raw material , biopolymer , fourier transform infrared spectroscopy , nuclear chemistry , polyol , biomass (ecology) , yield (engineering) , organic chemistry , chemical engineering , bacteria , materials science , polymer , enzyme , biology , hydrolysis , ecology , agronomy , genetics , metallurgy , polyurethane , engineering
Biopolymers are a new generation biopolymers, which have wide range of applications. Poly-3-hydroxybutyrate (PHB) is one of the best biopolymers for replacement of non-biodegradable petroleum-based plastic and can be produced from organic wastes source by some bacterial strains under imbalance growth conditions. In present study, hydrolyzate was prepared from biomass of seaweed Ulva by acid pre-treated and used as a feedstock for PHB biosynthesis by Bacillu subtilis rnM. The pre-treatment was carried out by two different pre-treatment conditions such as room temperature and high temperature and pressure (HTP). The hydrolysate prepared by 2 % HCl at HTP supported for the maximum PHB biosynthesis than the other pre-treated conditions. The yield of PHB obtained by B. subtilis rnM when tested with laboratory grade sugars was lower to that achieved with hydrolysate of Ulva sp. The biosynthesized PHB was characterized by TGA, DSC, FTIR, XRD and NMR techniques.