Open AccessSimultaneous Estimation of Paracetamol, Phenylephrine Hydrochloride and Triprolidine Hydrochloride by Second Order Derivative Spectroscopy
Author(s) -
Mukem Bhattarai,
Hema Basnett,
Ateeb Das,
Pravat Manna,
Debarupa Dutta,
Deepak Chaudhary,
Bhupendra Shrestha
Publication year - 2019
Publication title -
asian journal of chemistry/asian journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.145
H-Index - 34
eISSN - 0975-427X
pISSN - 0970-7077
DOI - 10.14233/ajchem.2019.22244
Subject(s) - chemistry , chromatography , phenylephrine , hydrochloride , dosage form , derivative (finance) , relative standard deviation , detection limit , organic chemistry , medicine , blood pressure , financial economics , economics , radiology
A simple, sensitive and reproducible second order derivative UV spectrophotometric method was developed for simultaneous estimation of mixture of paracetamol, phenylephrine hydrochloride and triprolidine hydrochloride in the tablet dosage form. The method utilizes second order derivative technique, which helps to isolate the individual peaks of the mixture drugs and overcome the problem of merging of mixture peaks with each other. For quantification of paracetamol, phenylephrine hydrochloride and triprolidine hydrochloride wavelength selected were 244, 276 and 297 nm, respectively. The method was linear over the concentration range of 5-40 μg/mL. Intra-day and inter-day precision were within acceptable range (percentage relative standard deviation 2.27 %). The percentage recovery was within the range between 97.22-99.43 %. The method was also found to be robust and rugged. The method stands validated as per ICH guidelines and hence, can be used for the routine quality control analysis of paracetamol, phenylephrine hydrochloride and triprolidine hydrochloridein the tablet dosage form.