Open AccessQuantification of Prochlorperazine and Paracetamol Using High Performance Liquid Chromatography: Application to Tablets and Stability Studies
Author(s) -
Kadali Jagadeesh,
Annapurowduri
Publication year - 2019
Publication title -
asian journal of chemistry/asian journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.145
H-Index - 34
eISSN - 0975-427X
pISSN - 0970-7077
DOI - 10.14233/ajchem.2019.22140
Subject(s) - prochlorperazine , chemistry , chromatography , relative standard deviation , high performance liquid chromatography , detection limit , anesthesia , medicine , nausea
This study reports a new stability indicating HPLC method using Spursil C18 column as stationary phase, and mixture of 0.1 M Na2HPO4 and methanol (50:50 v/v) as mobile phase for the chromatographic determination of paracetamol and prochlorperazine in tablets and in bulk form. The linearity range is 250-750 μg/mL for paracetamol and 2.5-7.5 μg/mL for prochlorperazine. The limit of detection values are 2.650 μg/mL for paracetamol and 0.175 μg/mL for prochlorperazine. The minor values of the relative standard deviation (≤ 2.0 %) as well as good percent assay values (nearer to 100 %) confirm the high precision and accuracy of the present method. From the degradation study chromatograms found that there was no interference from degradants when paracetamol and prochlorperazine are quantified in tablets through the proposed method. A good agreement between results obtained and labeled claim for the determination of paracetamol and prochlorperazine in tablet samples demonstratesthat the proposed method is appropriate to quantify paracetamol and prochlorperazine in tablet formulations.