Open AccessHigh Performance Liquid Chromatography Analysis Using Rutin Marker and Estimation of Phenolic & Flavonoid Compounds in the Extracts of Indian Medicinal Plant Morus nigra L.
Author(s) -
Sarva Jit Singh,
Alka Tripathi,
V. K. Lal,
Dhananjay Singh
Publication year - 2019
Publication title -
asian journal of chemistry/asian journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.145
H-Index - 34
eISSN - 0975-427X
pISSN - 0970-7077
DOI - 10.14233/ajchem.2019.22003
Subject(s) - rutin , gallic acid , chemistry , flavonoid , quercetin , moraceae , high performance liquid chromatography , chromatography , antioxidant , traditional medicine , biochemistry , medicine
Morus nigra L. (black mulberry) belongs to moraceae family of Indian medicinal plants. Black mulberry has significant anticancerous, antioxidant, antidiabetic, antimicrobial and antiobesity activities. Rutin marker was used for high performance liquid chromatography technique. We used gallic acid and quercetin for phenolic compound determination and flavonoid content determination in plant leaf extract. Plant extract of this species was analyzed quantitatively and qualitatively through high pressure liquid chromatography. Rutin compound was the main marker, which is a flavonoidal compound. Standard solution (1 mg/mL) of rutin was prepared by using CH3OH. Methanol:water (80:20, v/v) ratio was used to dissolve powdered M. nigra plant leaves (100 mg), in which 0.01 mg/g rutin content was found. Total phenolic content in M. nigra was found 43.15 ± 0.68 mg/g GAE (gallic acid equivalents). Total flavonoid content in M. nigra was found 5.8 ± 0.46 mg/g QE. It was found that the M. nigra has not significant rutin potency and found to be as 0.01 mg/g.