Open AccessValidated UV-Vis Spectrophotometric Determination of Andrographolide in Herbal Nano-Preparation
Author(s) -
Indah Hairunisa,
Muhammad Da’i,
Erindyah Retno Wikantyasning,
Andhika Rizky Gilang Mahaputra,
Normaidah Normaidah,
Mohd Fadzelly Abu Bakar
Publication year - 2019
Publication title -
asian journal of chemistry/asian journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.145
H-Index - 34
eISSN - 0975-427X
pISSN - 0970-7077
DOI - 10.14233/ajchem.2019.21951
Subject(s) - andrographolide , detection limit , andrographis paniculata , chemistry , chromatography , relative standard deviation , reagent , methanol , spectrophotometry , phyllanthus , traditional medicine , organic chemistry , medicine , alternative medicine , pathology
Determination of major bioactive compounds in polyphyto-formulation is important for production of standardized herbal products. A fast, simple and inexpensive method for detection and quantification of andrographolide concentration in nanoemulsion preparations containing a combination of Andrographis paniculata (Burm f.) Ness. and Phyllanthus niruri L. has been developed. Detection and quantification were carried out using UV-vis spectrophotometry analysis with picric acid reagent and NaOH (8:2) in methanol solvents, read at maximum wavelength 479 nm with 22 min of incubationtime. Validation was done by determine the parameters such as linearity, intra and interday precision, accuracy, limit of detection (LOD) and limit of quantification (LOQ). The results obtained showed linearity with r = 0.9945 (y = 0.0109x − 0.2066) in the range of 30-80 μg/mL. The accuracy (recovery) varied in the range of 97.15 to 104.42 %. Percentage of relative standard deviation (% RSD) for precision and intermediate precision value were 3.23 and 3.02 % with LOD value 211 μg/mL and the LOQ 705 μg/mL. As a conclusion, this method is suitable to detect andrographolide content in herbalnano-preparation.