Open AccessBox-Behnken Experimental Design for Extraction of Spinosin from Ziziphus mauritiana Lam. Seeds
Author(s) -
Quang Thuong Tran,
Loan Thi Thanh Vu,
Tram Huyen Le,
Ly Thi Phuong Giang,
Toyonobu Usuki
Publication year - 2019
Publication title -
asian journal of chemistry/asian journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.145
H-Index - 34
eISSN - 0975-427X
pISSN - 0970-7077
DOI - 10.14233/ajchem.2019.21763
Subject(s) - box–behnken design , ziziphus , mauritiana , extraction (chemistry) , chemistry , chromatography , response surface methodology , botany , biology
The objective of this study was to evaluate the effects of extraction parameters (ethanol concentration, extraction ratio of solvent to raw material and extraction time) on the yield of spinosin from the seeds of Ziziphus mauritiana Lam. Box-Behnken factorial design was used in association with response surface methodology. The optimal extraction conditions were an ethanol concentration of 61 %, 40 (v/w) liquid-to-solid ratio, and 2.6 h extraction time with reflux. Spinosin was purified using Diaion HP20SS as an adsorbent. The maximum spinosin yield was 1.18 mg/g raw material. Present findings can be applied to future approaches for maximizing the extraction of spinosin from Z. mauritiana Lam. seeds.