Open AccessAction of Ganoderma lucidum mycelial growth filtrates on Erysiphe diffusa and embryotoxicity assessment in a chicken embryo model
Author(s) -
Mycheli Preuss da Cruz,
Lucas Trentin Larentis,
Edgar de Souza Vismara,
Lilian de Souza Vismara,
Paulo Fontoura Freitas,
Sérgio Miguel Mazaro
Publication year - 2021
Publication title -
acta biologica szegediensis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.212
H-Index - 28
eISSN - 1588-4082
pISSN - 1588-385X
DOI - 10.14232/abs.2021.1.47-57
Subject(s) - ganoderma lucidum , embryo , mycelium , embryogenesis , biology , antimicrobial , andrology , incubation , minimum inhibitory concentration , embryonic stem cell , spore , traditional medicine , microbiology and biotechnology , botany , food science , medicine , biochemistry , gene
This work aimed to evaluate the antimicrobial eect of Ganoderma lucidum mycelial growth filtrates (MGF) on the phytopathogen Erysiphe diffusa and their potential eects on the embryonic development of Gallus gallus. The antimicrobial activity was evaluated on E. diffusa spores by the microdilution broth method. To evaluate embryotoxic and teratogenic eects, fertile eggs of G. gallus received injections of solutions containing the filtrates of G. lucidum through the air chamber. After three days of incubation, we opened the eggs and evaluated egg viability, embryo survival, malformation occurrence, embryonic staging and heart rate. Live embryos were prepared using whole mount technique and the morphological analysis was performed. We used the generalized linear model to fit embryotoxicity and teratogenicity data. We verified that G. lucidum MGF showed inhibitory activity in vitro against E. diffusa and the minimum inhibitory concentrations ranged from 5 to 10 mg/mL. We could also observe that the filtrates did not present embryotoxic or teratogenic eects on the early embryonic development of G. gallus, but induced significant dierences in the embryonic mean heart rate and on the stage of embryonic development.