Open AccessPATOGENISITAS ISOLAT BAKTERI Xanthomonas oryzae pv. oryzae DAN PEMANTAUAN PENYAKIT HAWAR DAUN BAKTERI PADA PADI GALUR ISOGENIK
Author(s) -
Yadi Suryadi,
Triny S. Kadir
Publication year - 2017
Publication title -
berita biologi/berita biologi
Language(s) - English
Resource type - Journals
eISSN - 2337-8751
pISSN - 0126-1754
DOI - 10.14203/beritabiologi.v16i2.2393
Subject(s) - xanthomonas oryzae , xanthomonas oryzae pv. oryzae , biology , virulence , blight , inoculation , pathogenicity , veterinary medicine , gene , population , microbiology and biotechnology , pathogen , horticulture , genetics , medicine , environmental health
Bacterial leaf blight (BLB) caused by Xanthomonas oryzae pv. oryzae (Xoo) is an important rice disease due to its high intensity that and the mereased durability of its resistant variety. This study was aimed to determine the pathogenicity of Xoo isolates, which obtained from four regions/districts in West Java on three rice near isogenic lines (NILs) containing a single resistance (R) gene; and to study reaction of rice NILs/ differential genotypes containing mixture of resistant genes to Xoo population under endemic areas. Out of 22 Xoo isolates have been collected and further determined by ELISA assay. Ten Xoo isolates were selected and inoculated to identify their pathogenicity on three NILs i.e., IRBB5 (xa5), IRBB7 (Xa7) and IRBB21 (Xa21). Pathogenicity test showed that most of isolates produced large lesion, and four virulence groups were identified. Phylogenetic analysis suggested that the dominant virulent isolates were widely distributed at several district in West Java. It was shown that 11 NILs exhibited high levels of resistant reaction to the predominant Xoo pathotypes in Cianjur, while 10 lines were susceptible. The single R gene (IRBB7, IRBB21 and Java14) exhibited lower BLB severity. The IRBB50, IRBB51, IRBB52, IRBB53 lines (carrying two R genes), IRBB56, IRBB57 (carrying three R genes); IRBB64 (carrying four R genes), and IRBB66 (carrying five R genes) showed lower severity and thus produced higher resistance to Xoo. This study may implied further work to deploy effective R genes against certain Xoo pathotypes in differ region.