Open AccessDevelopment and evaluation of a rapid CRISPR-based diagnostic for COVID-19
Author(s) -
Tieying Hou,
Weiqi Zeng,
Minling Yang,
Wenjing Chen,
Lili Ren,
Jingwen Ai,
Ji Wu,
Yalong Liao,
Xuejing Gou,
Yongjun Li,
Xiaorui Wang,
Hang Su,
Bing Gu,
Jianwei Wang,
Xu Teng
Publication year - 2020
Publication title -
plos pathogens
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.719
H-Index - 206
eISSN - 1553-7374
pISSN - 1553-7366
DOI - 10.1371/journal.ppat.1008705
Subject(s) - crispr , covid-19 , metagenomics , outbreak , computational biology , loop mediated isothermal amplification , biology , virology , molecular diagnostics , dna sequencing , diagnostic test , infectious disease (medical specialty) , medicine , bioinformatics , genetics , dna , gene , disease , pathology , emergency medicine
The recent outbreak of human infections caused by SARS-CoV-2, the third zoonotic coronavirus has raised great public health concern globally. Rapid and accurate diagnosis of this novel pathogen posts great challenges not only clinically but also technologically. Metagenomic next-generation sequencing (mNGS) and reverse-transcription PCR (RT-PCR) have been the most commonly used molecular methodologies. However, each has their own limitations. In this study, we developed an isothermal, CRISPR-based diagnostic for COVID-19 with near single-copy sensitivity. The diagnostic performances of all three technology platforms were also compared. Our study aimed to provide more insights into the molecular detection of SARS-CoV-2, and also to present a novel diagnostic option for this new emerging virus.