Open AccessSingle- and duplex TaqMan-quantitative PCR for determining the copy numbers of integrated selection markers during site-specific mutagenesis in Toxoplasma gondii by CRISPR-Cas9
Author(s) -
Kai Pascal Alexander Hänggeli,
Andrew Hemphill,
Norbert Müller,
Bernd Schimanski,
Philipp Olias,
Joachim Müller,
Ghalia Boubaker
Publication year - 2022
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0271011
Subject(s) - biology , taqman , dihydrofolate reductase , microbiology and biotechnology , toxoplasma gondii , amplicon , dna , real time polymerase chain reaction , polymerase chain reaction , virology , genetics , gene , antibody