Open AccessGene expression profile of chronic oral graft-versus-host disease
Author(s) -
Giselle Rocha Pinto,
Viviane Almeida Sarmento,
Paulo Cirino de Carvalho-Filho,
Vitor Fortuna,
Ryan dos Santos Costa,
Rogério Reis Conceição,
Soraya Castro Trindade
Publication year - 2022
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0267325
Subject(s) - pathogenesis , immunology , chemokine , hematopoietic stem cell transplantation , graft versus host disease , peripheral blood mononuclear cell , gene expression , stem cell , transplantation , medicine , biology , immune system , gene , genetics , in vitro
Among the complications observed after allogeneic hematopoietic stem cell transplantation, graft-versus-host disease (GVHD) is the primary cause of post-transplant mortality. The oral cavity is the second most affected organ target in chronic GVHD. Tissue damage results from the upregulation of inflammatory mediators, which play a critical role in the immunopathogenesis of the disease. This case series observational study aims to evaluate the participation of cytokines, chemokines, transcription factors, and heat shock proteins in the pathogenesis of oral GVHD (oGVHD), describing the mRNA expression of 28 genes selected. Peripheral blood mononuclear cells were isolated from six participants with oGVHD and two without GVHD, and relative expression of transcripts with established roles as inflammatory mediators was determined in triplicate using the human RT 2 Profiler™ PCR Array. The gene expression levels in the group with oGVHD were mainly up-regulated compared to those without GVHD. PBMC from oGVDH expressed consistently higher IFN-γ , TNF , IL-1β , CCL2 , HSP60 (HSPD1) and HSP90 (HSP90B1) . These results can provide a basis for developing new molecular diagnostics and targets therapies for the clinical management of oGVHD.