Open AccessAnalysis of multidrug efflux transporters in resistance to fatty acid salts reveals a TolC-independent function of EmrAB in Salmonella enterica
Author(s) -
Tomohiro Yoneda,
Hisanobu Sakata,
Seiji Yamasaki,
Mitsuko Hayashi-Nishino,
Kunihiko Nishino
Publication year - 2022
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0266806
Subject(s) - salmonella enterica , efflux , multiple drug resistance , salmonella , transporter , microbiology and biotechnology , biology , function (biology) , drug resistance , chemistry , biochemistry , bacteria , genetics , gene
Fatty acids salts exhibit bacteriostatic and bactericidal effects to inhibit bacterial growth and survival. Bacteria adapt to their environment to overcome these antibacterial effects through undefined mechanisms. In Gram-negative bacteria, drug efflux systems are associated with resistance to various substances. Studies have identified multiple drug efflux systems in Salmonella enterica . The aim of this study was to investigate whether drug efflux systems contribute to fatty acid salts resistance in S . enterica . We used deletion and overexpressing strains of S . enterica for drug efflux transporters. Susceptibility to fatty acid salts was determined by measuring minimum inhibitory concentrations and performing growth assays. Our findings revealed that acrAB , acrEF , emrAB and tolC in S . enterica contribute resistance to fatty acid salts. Furthermore, EmrAB, which is known to function with TolC, contributes to the fatty acid salts resistance of S . enterica in a TolC-independent manner. This study revealed that drug efflux systems confer fatty acid satls resistance to S . enterica . Notably, although EmrAB is normally associated with antimicrobial resistance in a TolC-dependent manner, it was found to be involved in fatty acid salts resistance in a TolC-independent manner, indicating that the utilization of TolC by EmrAB is substrate dependent in S . enterica .