Open AccessWavelengths and irradiances modulate the circadian rhythm of Neurospora crassa
Author(s) -
Peijun Wen,
Fuyun Tan,
Ming Lei,
Muhammad Saddique Akbar Khan,
Weihua Chen,
Xiaocheng Hu
Publication year - 2022
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0266266
Subject(s) - circadian rhythm , neurospora crassa , biology , rhythm , circadian clock , biophysics , chronobiology , medicine , endocrinology , genetics , gene , mutant
The circadian rhythm affects the biological evolution and operating mechanisms of organisms. The impact of light on the circadian rhythm is a significant concern for both biology and human well-being. However, the relation between different wavelengths, irradiances, and circadian rhythm is unknown. In this study, we compared the effects of four different monochromatic light-emitting diode (LED) light and two different irradiances on the circadian rhythm of a wild-type Neurospora crassa . The results demonstrated that the circadian rhythm of Neurospora crassa can be modulated by violet (λp = 393 nm), blue (λp = 462 nm), and green (λp = 521 nm) light, regardless of the irradiances, in the visible region. Unexpectedly, for the yellow light (λp = 591 nm), the 2 W/m 2 light had a more significant impact on circadian rhythm modulation than the 0.04 W/m 2 light had. Considering the highest energy of yellow light (2.25 eV) is lower than the High Occupied Molecular Orbital (HOMO)-Lowest Unoccupied Molecular Orbital (LUMO) gap of WC-1 (2.43 eV). We speculate that there may be other potential photoreceptors that are involved in circadian rhythm modulation. The HOMO-LOMO gaps of these proteins are greater than 1.98 eV and less than 2.25 eV. These results provide a strong foundation for a deeper understanding of the impact of different light on the circadian rhythm and also shed light on the identification of new circadian rhythm modulation photoreceptors.