Open AccessBioprospecting of fungal endophytes from Oroxylum indicum (L.) Kurz with antioxidant and cytotoxic activity
Author(s) -
N. Rai,
Priyanka Kumari Keshri,
Priyamvada Gupta,
Ashish Verma,
Swapnil C. Kamble,
Santosh Kumar Singh,
Vibhav Gautam
Publication year - 2022
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0264673
Subject(s) - dpph , antioxidant , hela , ec50 , ethyl acetate , plant use of endophytic fungi in defense , traditional medicine , biology , chemistry , botany , biochemistry , in vitro , medicine
Oroxylum indicum (L.) Kurz, a medicinal plant, shows numerous pharmacological properties which may be attributed to the bioactive compounds produced by O . indicum or due to associated endophytes. In the present study, leaf of O . indicum was evaluated for the presence of associated fungal endophytes, and antioxidant and cytotoxic activities of bioactive compounds produced from them. Using culture-dependent approach, eight fungal endophytes belonging to five different genera were identified. Two endophytes Daldinia eschscholtzii and Ectophoma multirostrata have been reported for the first time from the leaf of O . indicum plant. High-performance thin-layer chromatography (HPTLC) of ethyl acetate (EA) extract of isolated fungal endophytes showed a distinct fingerprinting profile in EA extract of Colletotrichum gloeosporioides . Among identified endophytes, EA extract of C . gloeosporioides showed significant antioxidant activity against DPPH free radical, superoxide anion radical, nitric oxide radical and hydroxyl radical with EC 50 values of 22.24±1.302 μg/mL, 67.46±0.576 μg/mL, 80.10±0.706 μg/mL and 61.55±1.360 μg/mL, respectively. EA extract of C . gloeosporioides exhibited potential cytotoxicity against HCT116, HeLa and HepG2 cancer cell lines with IC 50 values of 76.59 μg/mL, 176.20 μg/mL and 1750.70 μg/mL, respectively. A comparative HPTLC fingerprinting and the antioxidant activity of C . gloeosporioides associated with two different hosts (leaf of O . indicum and dead twigs of other plant) showed that C . gloeosporioides produces bioactive compounds in a host-dependent manner.