Open AccessSpatial redistribution of neurosecretory vesicles upon stimulation accelerates their directed transport to the plasma membrane
Author(s) -
Elaine B. Schenk,
Frédéric A. Meunier,
Dietmar Oelz
Publication year - 2022
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0264521
Subject(s) - vesicle , stimulation , biophysics , redistribution (election) , membrane , chemistry , intracellular , kinetics , vesicle fusion , microbiology and biotechnology , biology , biochemistry , endocrinology , synaptic vesicle , physics , quantum mechanics , politics , political science , law
Through the integration of results from an imaging analysis of intracellular trafficking of labelled neurosecretory vesicles in chromaffin cells, we develop a Markov state model to describe their transport and binding kinetics. Our simulation results indicate that a spatial redistribution of neurosecretory vesicles occurs upon secretagogue stimulation leading vesicles to the plasma membrane where they undergo fusion thereby releasing adrenaline and noradrenaline. Furthermore, we find that this redistribution alone can explain the observed up-regulation of vesicle transport upon stimulation and its directional bias towards the plasma membrane. Parameter fitting indicates that in the deeper compartment within the cell, vesicle transport is asymmetric and characterised by a bias towards the plasma membrane.