Open AccessChorioallantoic membrane (CAM) assay to study treatment effects in diffuse intrinsic pontine glioma
Author(s) -
Erica A Power,
Jenelys Fernandez-Torres,
Liang Zhang,
Ruiyi Yaun,
Fabrice Lucien,
David J. Daniels
Publication year - 2022
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0263822
Subject(s) - glioma , chorioallantoic membrane , radiation therapy , medicine , epigenetics , in vivo , cancer research , pathology , biology , angiogenesis , genetics , gene
Diffuse intrinsic pontine glioma (DIPG) is a lethal pediatric brain tumor. While there are a number of in vivo rodent models for evaluating tumor biology and response to therapy, these models require significant time and resources. Here, we established the chick-embryo chorioallantoic (CAM) assay as an affordable and time efficient xenograft model for testing a variety of treatment approaches for DIPG. We found that patient-derived DIPG tumors develop in the CAM and maintain the same genetic and epigenetic characteristics of native DIPG tumors. We monitored tumor response to pharmaco- and radiation therapy by 3-D ultrasound volumetric and vasculature analysis. In this study, we established and validated the CAM model as a potential intermediate xenograft model for DIPG and its use for testing novel treatment approaches that include pharmacotherapy or radiation.