Open AccessArrested crossover precursor structures form stable homologous bonds in a Tetrahymena meiotic mutant
Author(s) -
Miao Tian,
Kazufumi Mochizuki,
Josef Finsterer
Publication year - 2022
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0263691
Subject(s) - meiosis , chiasma , tetrahymena , homologous chromosome , anaphase , biology , homologous recombination , mutant , prophase , microbiology and biotechnology , genetics , bivalent (engine) , chromosomal crossover , dna , gene , chromosome , chemistry , organic chemistry , metal
Meiotic DNA double-strand breaks produce reciprocally exchanged DNA strands, which mature into chiasmata that hold homologous chromosomes together as bivalents. These bivalents are subsequently separated in the first meiotic division. In a mutant lacking the newly identified Tetrahymena gene APRO1 (Anaphase promoting 1), meiosis is arrested by the end of prophase. Mature chiasmata are not formed but bivalents are connected via a molecular precursor structure. In-depth analysis of this arrested intermediate structure may help to elucidate the noncanonical molecular recombination pathway in Tetrahymena .