Open AccessComparative metabolomics analysis of milk components between Italian Mediterranean buffaloes and Chinese Holstein cows based on LC-MS/MS technology
Author(s) -
Xiang Yuan,
Wen Shi,
Jianping Jiang,
Zhipeng Li,
Penghui Fu,
Chunyan Yang,
Saif ur Rehman,
Alfredo Pauciullo,
Qingyou Liu,
Deshun Shi
Publication year - 2022
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0262878
Subject(s) - metabolomics , conjugated linoleic acid , nicotinamide , metabolic pathway , uric acid , metabolism , food science , threonine , biology , metabolome , chemistry , tandem mass spectrometry , biochemistry , serine , linoleic acid , chromatography , fatty acid , mass spectrometry , enzyme
Buffalo and cow milk have a very different composition in terms of fat, protein, and total solids. For a better knowledge of such a difference, the milk metabolic profiles and characteristics of metabolites was investigated in Italian Mediterranean buffaloes and Chinese Holstein cows were investigated by liquid chromatography tandem-mass spectrometry (LC-MS/MS) in this study. Totally, 23 differential metabolites were identified to be significantly different in the milk from the two species of which 15 were up-regulated and 8 down-regulated in Italian Mediterranean buffaloes. Metabolic pathway analysis revealed that 4 metabolites (choline, acetylcholine, nicotinamide and uric acid) were significantly enriched in glycerophospholipid metabolism, nicotinate and nicotinamide metabolism, glycine, serine and threonine metabolism, as well as purine metabolism. The results provided further insights for a deep understanding of the potential metabolic mechanisms responsible for the different performance of Italian Mediterranean buffaloes’ and Chinese Holstein cows’ milk. The findings will offer new tools for the improvement and novel directions for the development of dairy industry.