Open AccessIgG3 and IgM Identified as Key to SARS-CoV-2 Neutralization in Convalescent Plasma Pools
Author(s) -
Christina Kober,
Sandro Manni,
Svenja Wolff,
Thomas W. Barnes,
Shatanik Mukherjee,
Thomas Vogel,
Lea Hoenig,
Vogel Peter,
Aaron Hahn,
Michaela Gerlach,
Martin Vey,
Eleonora Widmer,
Björn Keiner,
Patrick Schuetz,
Nathan Roth,
Uwe Kalina
Publication year - 2022
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0262162
Subject(s) - neutralization , convalescent plasma , antibody , virology , plasma protein binding , covid-19 , immunoglobulin g , receptor , in vitro , biology , immunoglobulin m , immunology , chemistry , medicine , infectious disease (medical specialty) , biochemistry , disease
Analysis of convalescent plasma derived from individuals has shown that IgG3 has the most important role in binding to SARS-CoV-2 antigens; however, this has not yet been confirmed in large studies, and the link between binding and neutralization has not been confirmed. By analyzing plasma pools consisting of 247–567 individual convalescent donors, we demonstrated the binding of IgG3 and IgM to Spike-1 protein and the receptor-binding domain correlates strongly with viral neutralization in vitro . Furthermore, despite accounting for only approximately 12% of total immunoglobulin mass, collectively IgG3 and IgM account for approximately 80% of the total neutralization. This may have important implications for the development of potent therapies for COVID-19, as it indicates that hyperimmune globulins or convalescent plasma donations with high IgG3 concentrations may be a highly efficacious therapy.