Open AccessAge-related changes in Kv4/Shal and Kv1/Shaker expression in Drosophila and a role for reactive oxygen species
Author(s) -
Maximiliano J. Vallejos,
Abdunaser Eadaim,
Eu-Teum Hahm,
Susan Tsunoda
Publication year - 2021
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0261087
Subject(s) - shaker , reactive oxygen species , drosophila (subgenus) , biology , drosophila melanogaster , microbiology and biotechnology , genetics , gene , physics , quantum mechanics , vibration
Age-related changes in ion channel expression are likely to affect neuronal signaling. Here, we examine how age affects K v 4/Shal and K v 1/Shaker K + channel protein levels in Drosophila . We show that K v 4/Shal protein levels decline sharply from 3 days to 10 days, then more gradually from 10 to 40 days after eclosion. In contrast, K v 1/Shaker protein exhibits a transient increase at 10 days that then stabilizes and eventually declines at 40 days. We present data that begin to show a relationship between reactive oxygen species (ROS), K v 4/Shal, and locomotor performance. We show that K v 4/Shal levels are negatively affected by ROS, and that over-expression of Catalase or RNAi knock-down of the ROS-generating enzyme, Nicotinamide Adenine Dinucleotide Phosphate (NADPH) Oxidase (NOX), can attenuate the loss of K v 4/Shal protein. Finally, we compare levels of K v 4.2 and K v 4.3 in the hippocampus, olfactory bulb, cerebellum, and motor cortex of mice aged 6 weeks and 1 year. While there was no global decline in K v 4.2/4.3 that parallels what we report in Drosophila , we did find that K v 4.2/4.3 are differentially affected in various brain regions; this survey of changes may help inform mammalian studies that examine neuronal function with age.