Open AccessProteomics and immunocharacterization of Asian mountain pit viper (Ovophis monticola) venom
Author(s) -
Siravit Sitprija,
Lawan Chanhome,
Onrapak Reamtong,
Tipparat Thiangtrongjit,
Taksa Vasaruchapong,
Orawan Khow,
Jureeporn Noiphrom,
Panithi Laoungbua,
Arissara Tubtimyoy,
Narongsak Chaiyabutr,
Supeecha Kumkate
Publication year - 2021
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0260496
Subject(s) - antivenom , venom , viper venoms , envenomation , snake venom , viper , disintegrin , viperidae , biology , chemistry , biochemistry , enzyme , metalloproteinase
The venomic profile of Asian mountain pit viper Ovophis monticola is clarified in the present study. Using mass spectrometry-based proteomics, 247 different proteins were identified in crude venom of O . monticola found in Thailand. The most abundant proteins were snake venom metalloproteases (SVMP) (36.8%), snake venom serine proteases (SVSP) (31.1%), and phospholipases A 2 (PLA 2 ) (12.1%). Less abundant proteins included L-amino acid oxidase (LAAO) (5.7%), venom nerve growth factor (3.6%), nucleic acid degrading enzymes (3.2%), C-type lectins (CTL) (1.6%), cysteine-rich secretory proteins (CRISP) (1.2%) and disintegrin (1.2%). The immunoreactivity of this viper’s venom to a monovalent antivenom against green pit viper Trimeresurus albolabris , or to a polyvalent antivenom against hemotoxic venom was investigated by indirect ELISA and two-dimensional (2D) immunoblotting. Polyvalent antivenom showed substantially greater reactivity levels than monovalent antivenom. A titer for the monovalent antivenom was over 1:1.28x10 7 dilution while that of polyvalent antivenom was 1:5.12x10 7 . Of a total of 89 spots comprising 173 proteins, 40 spots of predominantly SVMP, SVSP and PLA 2 were specific antigens for antivenoms. The 49 unrecognized spots containing 72 proteins were characterized as non-reactive proteins, and included certain types of CTLs and CRISPs. These neglected venom constituents could limit the effectiveness of antivenom-based therapy currently available for victims of pit viper envenomation.