Open AccessBacterial variation in the oral microbiota in multiple sclerosis patients
Author(s) -
Zahra Zangeneh,
Ahya Abdi-Ali,
Kianoosh Khamooshian,
Amirhoushang Alvandi,
Ramin Abiri
Publication year - 2021
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0260384
Subject(s) - veillonella , peptostreptococcus , microbiology and biotechnology , biology , fusobacterium , bacteroides , prevotella , temperature gradient gel electrophoresis , bacteria , bifidobacterium , population , saliva , microbiome , fusobacterium nucleatum , lactobacillus , porphyromonas gingivalis , streptococcus , medicine , 16s ribosomal rna , genetics , biochemistry , environmental health
Background Microorganisms in oral cavity are called oral microbiota, while microbiome consists of total genome content of microorganisms in a host. Interaction between host and microorganisms is important in nervous system development and nervous diseases such as Autism, Alzheimer, Parkinson and Multiple Sclerosis (MS). Bacterial infections, as an environmental factor in MS pathogenesis play role in T helper 17(Th17) increase and it enhancing the production of pro-inflammatory cytokines such as Interlukin-21(IL-21), IL-17 and IL -22. Oral microbiota consists diverse populations of cultivable and uncultivable bacterial species. Denaturing gradient gel electrophoresis (DGGE) is an acceptable method for identification of uncultivable bacteria. In this study, we compared the bacterial population diversity in the oral cavity between MS and healthy people. Methods From October to March 2019, samples were taken at Kermanshah University of Medical Sciences’ MS patients center. A total of 30 samples were taken from MS patients and another 30 samples were taken from healthy people. Phenotypic tests were used to identify bacteria after pure cultures were obtained. DNA was extracted from 1 mL of saliva, and PCR products produced with primers were electrophoresed on polyacrylamide gels. Results The genera Staphylococcus , Actinomyces , Fusobacterium , Bacteroides , Porphyromonas , Prevotella , Veillonella , Propionibacterium and uncultivable bacteria with accession number MW880919-25 , JQ477416 . 1 , KF074888 .1 and several other un-culturable strains were significantly more abundant in the MS group while Lactobacillus and Peptostreptococcus were more prevalent in the normal healthy group according to logistic regression method. Conclusion Oral micro-organisms may alleviate or exacerbate inflammatory condition which impact MS disease pathogenesis. It may be assumed that controlling oral infections may result in reduction of MS disease progression.