Open AccessIFNγ suppresses the expression of GFI1 and thereby inhibits Th2 cell proliferation
Author(s) -
Md. Murshed Hasan Sarkar,
Ryoji Yagi,
Yutaka Endo,
Ryo KoyamaNasu,
Yangsong Wang,
Ichita Hasegawa,
Toshihiro Ito,
Ilkka Junttila,
Jinfang Zhu,
Motoko Kimura,
Toshinori Nakayama
Publication year - 2021
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0260204
Subject(s) - cell growth , microbiology and biotechnology , repressor , biology , cell cycle , cytokine , cell , regulation of gene expression , transcription factor , chemistry , gene , immunology , genetics
While IFNγ is a well-known cytokine that actively promotes the type I immune response, it is also known to suppress the type II response by inhibiting the differentiation and proliferation of Th2 cells. However, the mechanism by which IFNγ suppresses Th2 cell proliferation is still not fully understood. We found that IFNγ decreases the expression of growth factor independent-1 transcriptional repressor (GFI1) in Th2 cells, resulting in the inhibition of Th2 cell proliferation. The deletion of the Gfi1 gene in Th2 cells results in the failure of their proliferation, accompanied by an impaired cell cycle progression. In contrast, the enforced expression of GFI1 restores the defective Th2 cell proliferation, even in the presence of IFNγ. These results demonstrate that GFI1 is a key molecule in the IFNγ-mediated inhibition of Th2 cell proliferation.