Open AccessMultilocus sequence analysis reveals different lineages of Pseudomonas anguilliseptica associated with disease in farmed lumpfish (Cyclopterus lumpus L.)
Author(s) -
Even Bysveen Mjølnerød,
Hanne Nilsen,
Snorre Gulla,
Andreas Riborg,
Kirsten Liland Bottolfsen,
Tom Wıklund,
Debes H. Christiansen,
Jesús L. Romalde,
Felix Scholz,
Duncan J. Colquhoun
Publication year - 2021
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0259725
Subject(s) - biology , genotype , rpob , multilocus sequence typing , phylogenetic tree , population , housekeeping gene , zoology , microbiology and biotechnology , genetics , 16s ribosomal rna , bacteria , gene , gene expression , demography , sociology
The bacterium Pseudomonas anguilliseptica has in recent years emerged as a serious threat to production of lumpfish in Norway. Little is known about the population structure of this bacterium despite its association with disease in a wide range of different fish species throughout the world. The phylogenetic relationships between 53 isolates, primarily derived from diseased lumpfish, but including a number of reference strains from diverse geographical origins and fish species, were reconstructed by Multi-Locus Sequence Analysis (MLSA) using nine housekeeping genes ( rpoB , atpD , gyrB , rpoD , ileS , aroE , carA , glnS and recA ). MLSA revealed a high degree of relatedness between the studied isolates, altough the seven genotypes identified formed three main phylogenetic lineages. While four genotypes were identified amongst Norwegian lumpfish isolates, a single genotype dominated, irrespective of geographic origin. This suggests the existence of a dominant genotype associated with disease in production of lumpfish in Norwegian aquaculture. Elucidation of the population structure of the bacterium has provided valuable information for potential future vaccine development.