Open AccessEffect of Inonotus obliquus polysaccharide on composition of the intestinal flora in mice with acute endometritis
Author(s) -
Binhong Hu,
Yuqing Dong,
Wenjing Zhou,
Yichuan Ma,
Luyao Li,
Xianhua Fu,
Wenxuan Zhang,
Yuanyue Luo,
Jingyu Pu,
Xin Deng,
Rong Zhang,
Songqin Liu
Publication year - 2021
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0259570
Subject(s) - inonotus obliquus , endometritis , microbiology and biotechnology , flora (microbiology) , biology , gut flora , tumor necrosis factor alpha , proinflammatory cytokine , interleukin , polysaccharide , inflammation , bacteria , cytokine , immunology , food science , biochemistry , pregnancy , genetics
Inonotus obliquus Polysaccharide ( IOP ) is a large molecule extracted from Inonotus obliqus , a medicinal fungus, which has a wide range of biological activities and has been shown to be associated with inflammation. The purpose of this study is to investigate whether IOP can help to reduce acute endometritis by regulating intestinal flora. We observed pathological changes in mice with endometritis following treatment with IOP and evaluated changes in the levels of interleukin-6 (IL-6), interleukin-1β (IL-1β) and tumor necrosis factor α (TNF-α), and further studied the effects of IOP on the intestinal flora of endometritis mice using 16S rRNA high-throughput sequencing. The results showed that IOP improved the condition of uterine tissues and reduced the release of pro-inflammatory cytokines. Meanwhile, the 16S rRNA sequencing results showed that IOP could regulate the changes in intestinal microflora at the level of genera, possibly by changing the relative abundance of some genera.