Open AccessTG2-gluten complexes as antigens for gluten-specific and transglutaminase-2 specific B cells in celiac disease
Author(s) -
Christian Borgen Lindstad,
Alisa E. Dewan,
Jorunn Stamnæs,
Ludvig M. Sollid,
M. Fleur du Pré
Publication year - 2021
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0259082
Subject(s) - tissue transglutaminase , gluten , antibody , antigen , cell , biology , microbiology and biotechnology , chemistry , immunology , enzyme , biochemistry
A hallmark of celiac disease is the gluten-dependent production of antibodies specific for deamidated gluten peptides (DGP) and the enzyme transglutaminase 2 (TG2). Both types of antibodies are believed to result from B cells receiving help from gluten-specific CD4 + T cells and differentiating into antibody-producing plasma cells. We have here studied the collaboration between DGP- and TG2-specific B cells with gluten-specific CD4 + T cells using transgenic mice expressing celiac patient-derived T-cell and B-cell receptors, as well as between B-cell transfectants and patient-derived gluten-specific T-cell clones. We show that multivalent TG2-gluten complexes are efficient antigens for both TG2-specific and DGP-specific B cells and allow both types of B cells to receive help from gluten-specific T cells of many different specificities.