Open AccessNeuroinductive properties of mGDNF depend on the producer, E. Coli or human cells
Author(s) -
Dzhirgala Shamadykova,
Dmitri Panteleev,
N. N. Kust,
Ekaterina Savchenko,
Ekaterina Yu. Rybalkina,
A. V. Revishchin,
Galina Pavlova
Publication year - 2021
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0258289
Subject(s) - glial cell line derived neurotrophic factor , neurotrophic factors , hek 293 cells , microbiology and biotechnology , gdnf family of ligands , dopaminergic , neuroscience , embryonic stem cell , cell culture , biology , neural stem cell , in vivo , in vitro , stem cell , dopamine , biochemistry , receptor , genetics , gene
The glial cell line‐derived neurotrophic factor (GDNF) is involved in the survival of dopaminergic neurons. Besides, GDNF can also induce axonal growth and creation of new functional synapses. GDNF potential is promising for translation to treat diseases associated with neuronal death: neurodegenerative disorders, ischemic stroke, and cerebral or spinal cord damages. Unproductive clinical trials of GDNF for Parkinson’s disease treatment have induced to study this failure. A reason could be due to irrelevant producer cells that cannot perform the required post-translational modifications. The biological activity of recombinant mGDNF produced by E . coli have been compared with mGDNF produced by human cells HEK293. mGDNF variants were tested with PC12 cells, rat embryonic spinal ganglion cells, and SH-SY5Y human neuroblastoma cells in vitro as well as with a mouse model of the Parkinson’s disease in vivo . Both in vitro and in vivo the best neuro-inductive ability belongs to mGDNF produced by HEK293 cells. Keywords: GDNF, neural differentiation, bacterial and mammalian expression systems, cell cultures, model of Parkinson’s disease.