Open AccessSpectral and time-resolved photoluminescence of human platelets doped with platinum nanoparticles
Author(s) -
Karina Matveeva,
Andrey Zyubin,
Elizaveta Demishkevich,
Vladimir Rafalskiy,
Ekaterina Moiseeva,
Igor Kon,
Anna Kundalevich,
Viktoria Butova,
И. Г. Самусев
Publication year - 2021
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0256621
Subject(s) - förster resonance energy transfer , quenching (fluorescence) , fluorescence , platinum , tryptophan , chemistry , quantum yield , tyrosine , photochemistry , platelet , biophysics , nanoparticle , amino acid , spectroscopy , membrane , materials science , nanotechnology , biochemistry , biology , physics , quantum mechanics , immunology , catalysis
This paper describes a detailed study of spectral and time-resolved photoprocesses in human platelets and their complexes with platinum (Pt) nanoparticles (NPs). Fluorescence, quantum yield, and platelet amino acid lifetime changes in the presence and without femtosecond ablated platinum NPs have been studied. Fluorescence spectroscopy analysis of main fluorescent amino acids and their residues (tyrosine (Tyr), tryptophan (Trp), and phenylalanine (Phe)) belonging to the platelet membrane have been performed. The possibility of energy transfer between Pt NPs and the platelet membrane has been revealed. Förster Resonance Energy Transfer (FRET) model was used to perform the quantitative evaluation of energy transfer parameters. The prospects of Pt NPs usage deals with quenching-based sensing for pathology’s based on platelet conformations as cardiovascular diseases have been demonstrated.