Open AccessNitrogen sources affected the biosynthesis of 2-acetyl-1-pyrroline, cooked rice elongation and amylose content in rice
Author(s) -
Pouwedeou Mouloumdema Potcho,
Nnaemeka Emmanuel Okpala,
Tchalla Korohou,
Muhammad Imran,
Nabieu Kamara,
Jisheng Zhang,
Kelvin Dodzi Aloryi,
Xiangru Tang
Publication year - 2021
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0254182
Subject(s) - amylose , panicle , cultivar , aromatic rice , agronomy , horticulture , elongation , biology , kharif crop , oryza sativa , chemistry , food science , crop , starch , biochemistry , materials science , ultimate tensile strength , gene , metallurgy
Many studies have been carried out on N sources effect on fragrant rice; however, their impact on rice grain quality is largely unclear. In this study, we evaluated the effects of different types of N sources on rice growth, yield, 2-acetyl-1-pyrroline (2AP), amylose and cooked rice elongation. Two indica rice cultivars, Basmati 385 (B385), Xiangyaxiangzhan (XYXZ) and two japonica cultivars, Yunjingyou (YJY), Daohuaxiang (DHX) were grown in experimental pots with six replications under four N sources: Potassium nitrate (KNO 3 ), ammonium bicarbonate (NH 4 HCO 3 ), urea (H 2 NCONH 2 ) and sodium nitrate (NaNO 3 ) in 2019 and 2020 early seasons. Our results showed that N dynamics regulated the number of panicles, 1000-grain weight, grain yield, 2-acetyl-1-pyrroline, amylose and cooked rice elongation across all the four treatments. The NH 4 HCO 3 treatment significantly increased the number of panicles and grain yield across the four rice varieties compared with KNO 3 , H 2 NCONH 2 and NaNO 3 N sources in both 2019 and 2020 early season, The KNO 3 treatment significantly showed higher 1000-grain weight in B-385, YJY, XYXZ and DHX compared to other N sources. Compared with other N sources treatment, the NH 4 HCO 3 treatments significantly increased the 2AP contents in heading stage leaves, matured leaves and grains of B-385, YJY, XYXZ and DHX respectively. Cooked rice elongation percentage also showed significant difference in all treatments studied with KNO 3 recorded the highest across the four varieties. Analysis of major enzymes and compounds such as P5C, P5CS, PDH, Pyrroline, proline and Methylglyoxal showed remarkable differences in each cultivar at heading and maturity stages with higher activity in NH 4 HCO 3 and H 2 NCONH 2 treatments. Similarly, in all treatments, we also observed significant increase in amylose content percentage, with NH 4 HCO 3 having greater percentage of amylose.