Open AccessSingle-cell transcriptome analysis of the zebrafish embryonic trunk
Author(s) -
Sanjeeva Metikala,
Satish Casie Chetty,
Saulius Sumanas
Publication year - 2021
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0254024
Subject(s) - transcriptome , zebrafish , biology , embryonic stem cell , cell type , computational biology , single cell analysis , developmental biology , microbiology and biotechnology , cell , cellular differentiation , cell fate determination , genetics , gene , gene expression , transcription factor
During embryonic development, cells differentiate into a variety of distinct cell types and subtypes with diverse transcriptional profiles. To date, transcriptomic signatures of different cell lineages that arise during development have been only partially characterized. Here we used single-cell RNA-seq to perform transcriptomic analysis of over 20,000 cells disaggregated from the trunk region of zebrafish embryos at the 30 hpf stage. Transcriptional signatures of 27 different cell types and subtypes were identified and annotated during this analysis. This dataset will be a useful resource for many researchers in the fields of developmental and cellular biology and facilitate the understanding of molecular mechanisms that regulate cell lineage choices during development.