Open AccessImbalanced dietary intake alters the colonic microbial profile in growing rats
Author(s) -
Tae-Hwan Jung,
Kyoung-Sik Han
Publication year - 2021
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0253959
Subject(s) - biology , butyrate , akkermansia , lactobacillus , feces , food science , bacteria , gut flora , akkermansia muciniphila , clostridium , short chain fatty acid , microbiology and biotechnology , microbiome , bifidobacterium , prevotella , biochemistry , fermentation , bioinformatics , genetics
An imbalanced dietary intake is associated with alteration of intestinal ecosystem. We investigated the impact of imbalanced diets on colonic microbiota, concentrations of short chain fatty acid in colonic digesta and serum immunoglobulins (Igs) of growing rats. Compared to the control diet, consuming diets high in fat, sucrose, or processed meat, or low in iron, increased the abundance of the pathogenic bacteria such as Clostridium , Escherichia coli , and Salmonella species, and decreased the beneficial bacteria, like Bifidobacteria , Lactobacillus , Akkermansia , Phascolarctobacterium , Alistipes , and butyrate producing species of bacteria in the colon of growing rats. The heatmap of metagenomics indicated that each group was separated into distinct clusters, and the ID group in particular, showed significantly ( P < 0.01) reduced alpha diversity of colonic microbiota in comparison to the control group. All experimental groups showed significantly ( P < 0.05 or P < 0.01) decreased concentration of acetate and butyrate in the colonic digesta and lower levels of serum IgG or IgA, compared to the control. These results indicated that the imbalanced dietary intake negatively altered intestinal ecosystem and immunity.