Open AccessProduction and сharacterization of the exopolysaccharide from strain Paenibacillus polymyxa 2020
Author(s) -
Elena V. Liyaskina,
Nadezhda A. Rakova,
Alevtina A. Kitykina,
Valentina V. Rusyaeva,
Filip V. Toukach,
Alexey Fomenkov,
Saulius Vainauskas,
Richard J. Roberts,
В. В. Ревин
Publication year - 2021
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0253482
Subject(s) - paenibacillus polymyxa , levansucrase , paenibacillus , chemistry , industrial microbiology , biology , biochemistry , microbiology and biotechnology , fermentation , bacillus subtilis , gene , bacteria , genetics , 16s ribosomal rna
Paenibacillus spp. exopolysaccharides (EPSs) have become a growing interest recently as a source of biomaterials. In this study, we characterized Paenibacillus polymyxa 2020 strain, which produces a large quantity of EPS (up to 68 g/L),and was isolated from wasp honeycombs. Here we report its complete genome sequence and full methylome analysis detected by Pacific Biosciences SMRT sequencing. Moreover, bioinformatic analysis identified a putative levan synthetic operon. Sac C and sac B genes have been cloned and their products identified as glycoside hydrolase and levansucrase respectively. The Fourier transform infrared (FT-IR) and nuclear magnetic resonance (NMR) spectra demonstrated that the EPS is a linear β-(2→6)-linked fructan (levan). The structure and properties of levan polymer produced from sucrose and molasses were analyzed by FT-IR, NMR, scanning electron microscopy (SEM), high performance size exclusion chromatography (HPSEC), thermogravimetric analysis (TGA), cytotoxicity tests and showed low toxicity and high biocompatibility. Thus, P . polymyxa 2020 could be an exceptional cost-effective source for the industrial production of levan-type EPSs and to obtain functional biomaterials based on it for a broad range of applications, including bioengineering.